Laying down boundaries for brain evolution in cichlids

Walter Garstang famously said that ontogeny creates phylogeny: you need to understand the development of a structure to understand the diversity of that structure across species.

There are a few different ways to change the way a structure is put together. Research on the development of limbs has tended to view morphological changes as being caused by changing boundaries that delineate different regions of the embryo. If you want a bigger forebrain, shift the boundary between the forebrain and the midbrain backward.

Neurobiologists, however, have sometimes suggested that brains differ due to the amount and duration of neurogenesis that goes on during development. If you want a bigger forebrain, make neurons for a longer time in the forebrain than in the midbrain.

A new paper by Sylvester and colleagues tries to look at the relative importance of these two developmental mechanisms in brain evolution. To do this, they looked at the brains of cichlid fishes. Cichlids are renowned among evolutionary biologists for their diversity and the speed at which it was achieved, and this diversity extends to their brain morphology. They chose six species to study; Cynotilapia afra and Copadichromis borleyi are shown here (click to enlarge). The former is a mbuna, a rock-dwelling fish, and the latter is a sand-dweller; the authors had three species of each.

The brains of the mbuna and non-mbuna differ in their proportions: the mbuna have smaller forebrains (strictly speaking, purists would say the telencephalon) than the non-mbuna.

Sylvester and colleagues visualized the cichlid brains using several that are well-known to be involved in development, like sonic hedgehog (shh), and Wnt and found that the pattern of expression of these did indeed differ in these species, typically following the mbuna / non-mbuna split.

To test whether these genes caused the differences in brain sizes, the authors exposed embryos of the mbuna species Labeotropheus fuelleborni (right) to lithium chloride, which affects the Wnt pathway. When they did this, they found that the L. fuelleborni brains were looking rather more like non-mbuna brains, which proportionately larger telencephalons.

Sylvester and company argue that this suggests the very early expression of genes that lay out patterns in the nervous system are key regulators of brain evolution. Considering that they sort of set up timing of neurogenesis as an alternate hypothesis in their introduction, however, it’s worth pointing out that they didn’t actually measure neurogenesis or try to manipulate it in a manner similar to what they did for these patterning genes and proteins. It’s possible that neurogenesis is playing a role in shaping diversity of brains in other ways than tested here.

Additional: The journal is publishing a commentary on this paper. While the original article is open access, the commentary, alas, is not.

Reference

Sylvester, J., Rich, C., Loh, Y., van Staaden, M., Fraser, G., & Streelman, J. (2010). Brain diversity evolves via differences in patterning Proceedings of the National Academy of Sciences 107(21): 9718-9723. DOI: 10.1073/pnas.1000395107

Photo of Cynotilapia afra by Calwhiz  on FLickr; photo of Labeotropheus fuelleborni by Lee Nachtigal on Flickr; photo of Copadichromis borleyi by Petrichor on Flickr. All used under a Creative Commons license.

Global warming, ocean acidification, and KO’d crayfish

What do global warming, ocean acidification, and KO’d crayfish have in common? Carbon dioxide.

A forthcoming paper by Biewbower and Cooper caught my eye because it incidentally supports a contention in the last paper I co-authored on crustacean nociception (Puri & Faulkes 2010). We couldn’t find any evidence that crustaceans responded to acids.

The new paper isn’t actually interested in acids; it’s all about what carbon dioxide does to crayfish. The first question is, can crayfish detect changes in carbon dioxide concentrations at all?

The authors ran crayfish through a Y-maze: one arm with high CO₂ concentrations, and one without. The higher the carbon dioxide concentrations, the more the crayfish tended to avoid that end of the maze, suggesting that yes, the crayfish can detect carbon dioxide in water. They also found that when placed in a pool containing high levels of carbon dioxide, crayfish would leave the water to cross in the air to a chamber with lower levels.

Another possible interpretation is that the crayfish aren’t detecting carbon dioxide in these experiments, but a lack of oxygen. It could also be low pH. When you ramp up the concentration of carbon dioxide in water, create carbonic acid. This is why burning fossil fuels is leading to ocean acidification. Crayfish could have avoided the end of the maze because they detected the low oxygen or low pH rather than the carbon dioxide specifically.

Consequently, they created two control conditions in addition to regular water. First, they created a low-oxygen water by bubbling nitrogen through it. Crayfish did not discriminate between the nitrogen laden water and the regular water. Second, they made one end of the Y maze with normal CO₂ concentrations, but acidic pH; about 4.85.

And the crayfish walked right into it.

No difference between the regular water and the acidic. So their study supports ours: decapod crustaceans seem to be mostly ignorant of changes in pH.

Another interpretation might be that the animals are responding to low oxygen; to control for that, the authors also had a control condition with very high nitrogen concentrations in the water, which lowered oxygen, and again, the crayfish didn’t distinguish between that and regular water.

Bierbower and Cooper also found that at high concentrations, carbon dioxide does a number on the crayfish, effectively putting them out of action stopping their heart and ventilation (“breathing,” more or less).

Why are crayfish repelled by high levels of carbon dioxide? Not clear. Bierbower and Cooper suggest that high levels of carbon dioxide might be a cue that an environment is toxic. That said, these are swamp crayfish (Procambarus clarkii), and they probably have a pretty high tolerance for low quality water. It would be interesting to see if crayfish that favour highly oxygenated streams might be more sensitive to carbon dioxide concentrations.

While very interesting to me because of its relevance to a story I’ve been working on (crustacean nociception), this paper might have limited interest to others. Both the introduction and discussion are very general, and don’t convey a strong sense of where this fits into a broader research picture.

References

Bierbower, S., & Cooper, R. (2010). The effects of acute carbon dioxide on behavior and physiology in Procambarus clarkii. Journal of Experimental Zoology Part A: Ecological Genetics and Physiology DOI: 10.1002/jez.620

Puri S, Faulkes Z. 2010. Do decapod crustaceans have nociceptors for extreme pH? PLoS ONE 5(4): e10244. doi: 10.1371/journal.pone.0010244

The unread masses of scientific literature

Canadian Girl Postdoc resumes her look at slow science. She asks:

Although I’ve heard the common refrain from faculty that some members of a search committee do look for quality, I wonder how many of them have actually read any of the papers from the job candidates. And if that assessment of quality is based on the journal’s impact factor or the faculty member's own assessment of the candidate's science?

Speaking just for me as a search committee member, I haven’t read papers by job applicants. First, it takes more time than I’m willing to give, and we tend not to have the problem of triple digit numbers of job applications.

Second, we are routinely hiring outside of areas that I am qualified to judge the technical proficiencies of the science. Heck, in many cases, we’re get applications from people whose science I almost literally do not understand at all.

I judge the research of job applicants largely by the number of publications, the titles of their articles, and their research statement, and yes, there’s a tiny little bit of journal “quality.” Taken together, those provide an overview of the kind of research someone is doing, and the questions they are asking.

Journal “quality” based on a very general perception. I don’t look up impact factors. More often, there’s someone on the search committee who knows about the journals people have published in, and weighs in with, “That’s a good, solid, international journal,” or, “Not so good.” Journal titles can reveal a lot about their scope, so you probably don’t want to publish in the Chinese Journal of Irreproducible Crap.

What about the whispers that a job applicant must have a “SNC” (Science, Nature, Cell) paper to be competitive? Obviously, a Science or Nature paper is a nearly universally known entity, so it helps, but it has never been a determining factor in our searches, or even a large factor. A consistent record in strong disciplinary journals has worked for us.

But you know what’s worse than me not reading the papers of our tenure track job candidates?

I rarely even read the papers of my own friends.

I know about the science of people in my department, or the ones I hang out with at conferences from talking to them in the hallways, not from reading their papers front to back.

I don’t read them for a lot of the same reasons I don’t read papers from job candidates: takes a lot of time, their research is far afield from mine, and I have other ways of learning what they’re doing.

I suppose the thing that makes it okay is that I know that they don’t read mine, either.

Save the Stazione Zoologica Anton Dohrn

I received the following email from Roberto Di Lauro, president of Stazione Zoologica Anton Dohrn.

The Stazione Zoologica Anton Dohrn of Naples is at risk of being closed by the Italian Government.

If you agree in signing the petition reported below, please go to http://www.szn.it click on "sign the petition" and fill in the form.

We learned that the Stazione Zoologica Anton Dohrn di Napoli will be suppressed according to the Decree-Law adopting urgent measures on financial stabilization and economic competitiveness, that the Italian Government is in the process of final approval.

The Stazione Zoologica, founded 130 years ago by Anton Dohrn, is among the most prestigious Italian Research Institutes. It acts in the field of marine biological research and is known for the multidisciplinary nature of its activities and for the excellent scientific output.

Current research activities, international and national collaborations, exchanges with scientists of different nationalities, and participation in numerous scientific projects in Europe and worldwide are a clear sign of the nature of the Stazione as an international research institution.

The Stazione Zoologica coordinates the project EMBRC, the only European infrastructure in the biomedical sciences coordinated by an Italian Institution and involving 14 institutions from seven European countries. The EMBRC project is a clear testimony of how the international spirit of the Institute is still alive and present.

Active participation of distinguished scientists, including three Nobel Laureates, as members of the Scientific Council of the Stazione is another demonstration of the relevance of the Institution among the international scientific community.

We manifest our serious concern for the suppression of the Stazione Zoologica, which would greatly reduce the impact of the Italian presence in the international scientific community.

We therefore ask that the Stazione is maintained as an autonomous structure. It is the autonomy, uniqueness, diversity and freedom of research that allowed the Stazione Zoologica to reach its present level of excellence in science and will continue to be among the flagships of the Italian research in the world.

Data underload: Correlation of mood and precipitation, according to Garbage

For those who don’t get the reference...

Dingoes do detours, dogs don’t

A picture like this used to adorn the office door of some of my fellow graduate students:



The original picture (minus the labels) was taken from a general biology textbook to illustrate detour problems. We look at that and think, “That’s easy. Run around the post. Silly dog.”

Dogs turn out to be fairly bad at detour problems. Squirrels, I understand, solve such problems in a heartbeat, given that they have evolved to navigate complex three-dimensional environments as they leap from branch to branch on trees. But dogs and squirrels are so distantly related and have such different ecologies that one being better than the other doesn’t tell us all that much informative.

Dogs, for instance, are domesticated animals. Domestication is often thought to make animals... well... stupid. Domestic animals often have smaller brains than their wild counterparts. Further, domestic dogs have social relationships with their owners; dogs tend to look at their owners when they experience problems solving a problem.

Smith and Litchfield pick up on some previous studies that showed wolves tended to do better on detour tasks than domestic dogs. They predicted that the same should be true of another kind of wild dog, Australian dingoes. They also predicted that dingoes would not look at humans when solving the problem.

To make a long story short, the dingos breezed through the tasks, and having humans around provided no particular advantage. Indeed, only one dingo ever even looked at a human when doing the task.

Are dingoes the super-geniuses of the dog world? The authors don’t think that that dingoes are especially bright; they really do think dogs have been made dumber by domestication. They write:

It is unclear whether domestic dogs have lost the ability to work independently to solve problems, or whether they are simply hindered or handicapped by their close relationship with humans.

But before closing out, I have to note that “domestic” doesn’t always mean “dumb” in spatial abilities. A little earlier this year, Lewejohann and colleagues tested domestic guinea pigs in the Morris water maze, a standard learning task for small mammals, against wild cavies, the original stock guinea pigs arose from. The authors here mention in the abstract that guinea pigs have smaller brains than cavies (but provide no reference – grrr).

To once again cut a long story short, the guinea pigs did better at learning the Morris water maze task than the cavies.

Of course, there are differences in these two spatial tasks. Traditionally, the detour task is thought to require some level of insight, while the Morris water maze doesn’t. But the guinea pig study certainly helps to show you can’t assume all the domestic animals are going to do worse than their wild counterparts.

Even if that is the case for dogs and dingoes.

Reference

Smith, B., & Litchfield, C. (2010). How well do dingoes, Canis dingo, perform on the detour task? Animal Behaviour. DOI: 10.1016/j.anbehav.2010.04.017

Lewejohann, L., Pickel, T., Sachser, N., & Kaiser, S. (2010). Wild genius - domestic fool? Spatial learning abilities of wild and domestic guinea pigs Frontiers in Zoology: 7 (1). DOI: 10.1186/1742-9994-7-9

Picture by Jared Kelly on Flickr, used under a Creative Commons license.

Students JAQing off in class

I know, I know. Even I’m getting tired of me fisking Texas State Board of Education members when they say dubious things about the teaching of evolution.

But for a change, this time it’s not Don McLeroy! Instead, it’s Ken Mercer (pictured), who is interviewed as part of his re-election campaign in District 5.

What is your position on the teaching of evolution?

I get upset when the newspapers say we put religion in the textbooks. It’s a lie. I challenge every editorial board to go online—the standards are online—and find religion in astronomy, chemistry, biology. It is not there.

Mercer is correct. There is no explicit religion in the science standards, although I note he gives no examples of newspapers that have said this. I have not seen any newspapers that claimed this. (I have seen some make mistakes, and have pointed them out.)

What I have read repeatedly is that State Board of Education’s relentless copy-editing of the standards is religiously motivated. I wonder if Mercer would dispute this. I doubt anyone can build a serious case that things like removing references to the estimated age of the universe were done for secular reasons.

I firmly believe kids in America have the right to raise their hands in the classroom and ask honest questions. I was rather shocked that the opposition didn’t want that. It wasn’t just evolution; it was global warming. I was shocked that in America if you’re either for or against those things, the biggest thing is they didn’t want kids to raise their hands and ask honest questions.

Ah, yes.

“Just asking questions.”

Sounds fair-minded. Who will object to students asking questions?

I doubt anyone is concerned about genuine inquiries prompted by curiosity. But notice how Mercer singles out evolution and global warming. These are areas where people often don’t ask honest questions, but indulge in JAQing off. I like this definition of this tactic (to which I’ve added a little emphasis):

The act of asking leading questions to influence your audience, then hiding behind the defense that they’re “Just Asking Questions," even when the underlying assumptions are completely insane.

Second, Mercer skillfully creates the impression that the previous standards did not allow students to ask questions. But that’s not what standards do. Standards are intended to specify content, not the process in a classroom. And no standards ever could specify what students can or can’t ask.

I’ve snipped the rest of his answer to this question because Mercer goes on to repeat his arguments:

• Newspapers lie (with no actual examples given)
• Students should just be able to ask questions (particularly about things that conservative Christian Republicans don’t like, like evolution and global warming)

But I just wanted to point out this nice little throw-in near the end:

My biggest quote was, “If our kids do not have the freedom to raise their hands in science class and ask honest questions, then we are no longer living in the United States of America.”

I’m just surprised that he didn’t also say the new Texas education standards help support the troops.

Mercer picture from here.

3 Quarks Daily contest, 2010 version

3 Quarks Daily has started nominations for their second science blogging contest.

If you’ve read something you’ve liked on any science blog, consider nominating it. There’s not many perks for blogging, and a little recognition in the form of a nomination goes a long way.

In Tegrity

My university’s news page has a story on a lecture capture system called Tegrity today that features some quotes from me.

(I made a joke about this system before, but it’s quite good.)

Changing the will

A new report from the National Bureau of Economic Research argues that science policy is breaking. Reasons? Science is being done by larger and larger teams of older and older people who know less and less. The Chronicle of Higher Education has more commentary on this report.

I may have more to write about some of these issues later, but one thing leaped out at me:

Institutions such as government grant agencies, the patent office, the science education system, and the Nobel Prize come under a unified focus in this paper. In all cases, the question is how these institutions can change.

The comment about the Nobel prize changing is kind of weird, because the answer is pretty obviously, “It can’t – at least not very much.”

The way the Nobel prize works was laid out in Alfred Nobel’s will. Nobel’s particular selection of areas to reward has struck many as odd, and has led to the creation of at least one full-blown urban legend. (See also here.)

Until someone is able to revive Alfred Nobel ^*, and get him to change his will ^**, we’re pretty much stuck with the prizes as they are now.

Reforming the Nobel prize is rather pointless, As the professionalization of science and the increase in the number of people working in science means that the Nobel prize just isn’t a real goal for the vast majority of workings scientists out there. We have much bigger problems. This constant obsession with the Nobel prizes is unhealthy.

^* And if anyone could do that, they’d probably get the Nobel prize.

^** And if you do change the will, Al, a prize for biology would make a lot of sense.

Tuesday Crustie: Down in the underground

This week’s picture of a burrowing crayfish, Engaeus granulatus, is inspired by a recent radio essay by Susan Lawler, which talks about her adventures working with (or, mor accurately, trying to dig up) members of this genus. You can read a transcript here, but do yourself a favour and listen to the audio, if for no other reason than to hear her imitate the past president of the International Association of Astacology.

Picture from here.

Wolftime

I’m thinking of changing my profile picture. Maybe to something like this:

I’m considering this because of this quote in the Chronicle of Higher Education, reporting on the Texas K-12 social studies standards.

David Bradley, a Republican from Beaumont, made no secret of his distrust of college professors.

“We’ve done our job, but once these students step out of 12^th grade, they’ll be thrown to the wolves,” he told reporters after the vote.

Good news, Mr. Bradley! Thanks to your board’s hard work, fewer students than now are likely to graduate from university, so our corrupting influence will be minimized!

Perseus has really let himself go...

And Pegasus is looking pretty gray, too.

Scandalous, but...

My home country, Canada, decided to try to recruit senior, world-class scientists. And that’s good.

Through the Canada Excellence Research Chairs program, they got nineteen people to agree to pull up stakes move to Canadian universities and work there. That’s good.

All nineteen are men. That’s a scandal.

There’s not another way to put it. Not just for the agency, but for our discipline worldwide.

The Current has a good discussion on this, although I have to correct Wendy Robbins, who said (13:37 into the segment; emphasis added):

It's interesting, they're trying to find Nobel quality people, right? If you look at the history of the Nobel prizes, it's very interesting there, and I'm sure a lot has to do with two things: with more women going into different areas, but also with women’s work being recognized. You know, early on, Marie Curie won two Nobel prizes, right? Rosalind Franklin never did, because Watson and Crick took her research and they became famous for the double helix, but it was her work.

Rosalind Franklin died from cancer in 1958, and the Nobel Prize for the discovery of DNA structure was not awarded until 1962 (which Watson and Crick shared with Maurice Wilkins). The Nobel prize rules forbids posthumous awards, so Franklin could not have won the Nobel Prize. Whether she might have won it had she lived is anyone’s guess.

Much has been written about Franklin’s treatment by her colleagues, and there’s every reason to think much of it was unfair. Nevertheless, it’s wrong to say she didn’t win the Nobel prize because of it.

More comment by Canadian science show host Bob McDonald.

Additional: The Current revisits Rosalind Franklin’s legacy, with an interview with Brenda Maddox, who wrote the biography Rosalind Franklin, The Dark Lady of DNA.

Rise from the ashes

A while ago, while cleaning out a storage closet, I came across a couple of boxes filled with cassette tapes. I’ve been slowly converting them to mp3 files over the last couple of weeks. Almost every time I comment on Twitter of Facebook about digitising old cassette tapes, someone asks how I’m doing it. So maybe this will be of interest to a few blog readers...

It’s pretty simple: it’s just a tape deck with a USB interface and a bit of software. The software “EZ Audio Converter” is pretty simple, which is both blessing and curse. There’s about one important option for recording: automatically separate tracks or not. It does a fairly good job of recognizing tracks for basic rock and roll songs, but not great for things like spoken word recordings. sometimes you have to go in and use another audio editor to split tracks.

You can’t pick the audio format (you will get mp3 and you will like it!), no fiddling with equalizer settings, and it assumes you have iTunes software.

There are other ways of converting cassettes to digital audio files, but some of them require you still have a tape deck, and I imagine a lot of people got rid of theirs sometime around the turn of the century. This one is nice because it’s a all in one package.

I also have a USB turntable for digitising vinyl from the same manufacturer. I haven’t used it yet, because it requires more table space, and I haven’t found a place to put it.

I have gotten spoiled, though, by ripping CDs. You can rip a 70 minute CD in a few moments, but with tapes and vinyl, you have to play the whole thing through at regular time... It’s also fun to look at some of the graphics, particularly for cassette singles (which were short lived, ephemeral things).

It’s nice to get back some music that I thought I’d never hear again. Obscure Canadian pop from the heyday of MuchMusic or The Q. Except maybe in a dodgy YouTube video that you can’t put on an first gen iPod nano.

Are scientists lonely?

Jason Goldman started this post by asking, “Does science life engender loneliness?”

I said yes, for academic scientists, who I have had the most opportunities to observe. I don’t know if some of these things are as true for scientists who work outside of the traditional university system. Here are a few thoughts on why scientific careers might contribute to a feeling of loneliness.

The entire educational and career structure is very centered on individual achievement. You don’t get a doctorate for a group effort; it represents an individual’s unique contribution to the scientific discipline.

The number of academic positions in a field is typically small. This means that for much of your early career, you have to move often far away, often frequently. While I personally found there to be great opportunities in living in other places, there is definitely potential for terrible isolation when moving to a new location. And even when the moving stops, scientists might end up in locations far from what they consider home, and may experience cultural isolation.

Major decisions about your career success are made by anonymous strangers far from you, namely people who peer review your grants and publications. You can’t meet them, talk to them, or have conversations with them.

Then, you only have to look at the reams and reams of science blogs out there that talk about the pressure to do more. More publications, more students, more grant proposals. People are always talking about work / life balance, and making jokes like, “The best thing about being a professor is that you get to work whatever eighteen hours of the day you want.”

And the perverse thing is, in science, there is almost no point of diminishing returns on data collection. More time spent at the bench or desk means more data, more papers, more proposals. Time in the lab means less time for friendships, unless your friends are in the lab. Maybe even a long term relationship might develop from that...

Indeed, most people’s main ally in the fight against loneliness is a supportive partner. But academia’s response to husbands, wives, and partners is summed up by the phrase, “two body problem.” Yes, the emphasis is deliberate. When you have an oblique phrase like that, which most academics know, it’s a strong indicator that academia is not friendly to partners.

Jason pointed out that other professions work long hours. This is true, but in other jobs, people know what you do. The idea of grad student trying to explain their thesis research to mom and dad is a recurring joke (here’s one). A scientist might be in a department where she is the only one with the knowledge to seriously tackle a problem. A plant physiologist is probably not going to be able to get help from the virologist colleague down the hall on a research problem, even though they may be good colleagues.

And while the online science community can be very helpful, there’s still nothing like hallway collaborations. When you can get them.

So are all us scientists social orphans? No. But all these aspects of academic science makes the career indifferent to hostile to people’s abilities to form social relationships.

Additional: This post on Academic Ecology discusses the reality of being a spousal “accommodation,” as such things are often euphemistically known.

More additional: The World’s Fair asks how people respond when you say you’re a scientist in this post; see the discussion, too.

Stealin’ the glory

There’s a very cool story about argonaut octopuses making the news wire rounds, arising from this new paper. Summaries of this exciting science can be found at Not Exactly Rocket Science and New Scientist.

Then, there’s this story:

Just one problem.

That’s not an argonaut.

It’s a nautilus.

Note the resemblance of the shell to this:

And not to this:

It’s like reporting the results of a horse race with a picture of donkey.

Additional: Wow! It got fixed! Go go mob of angry cephalopod fans! And now there’s video, too!

Argonaut shell from mrpbps on Flickr. Nautilus from PacificKlaus on Flickr. Both are used under a Creative Commons license.

Tuesday Crustie: Oh, snap

Snapping shrimp (Alpheus macrocheles). Picture from here.

Favourite time of year

Today is the start of my favourite time of the year here. Classes and finals are done. Summer session classes don’t start for a few weeks. And even when summer classes start again, there’s not the usual load of other “stuff” going on to distract and split up time.

I suppose the time between the end of fall and the beginning of spring semesters also have some elements of that, but it’s not quite the same. The weather is a bit nicer now, sometimes. And there’s none of the franticness that sometimes accompanies the winter solstice.

I have been chugging away for the last week or so to get some new data. Some of it will go into a conference abstract that’s due today. I have several manuscripts that are near completion, and should be pushed out the door fairly fast. The last time I did this, back in December / January, has already given me two journal articles and a couple of smaller pieces to show for it. Here’s hoping I can repeat that success over the next couple of weeks.

Even so, I do have a surprising amount of administrative stuff on my calendar for this being a slow time, though. Heck, I have six meetings this week already.

Fruitbatgate

Dylan Evans has put up a YouTube video with some of the documents related to what is now being tagged, inevitably, “Fruitbatgate.” See yesterday’s post on this.

The video is nice, but a bit hard to read, so I’ve taken the liberty of making some screen grabs (click to enlarge).

The initial complaint:

Dr. Evans’s initial response:

Findings of Human Resources:

Letter from Evans to President Murphy:

President Murphy has no hesitation in disciplining Dr. Evans.

Dr. Evans tries to clear things up:

I may transcribe them later. Once again, a petition can be found here: http://www.ipetitions.com/petition/freedebate/

Additional: Demurelemur has links to the original complaint. Clearly, the issue is more complicated than it first appears, as most interpersonal matters leading to harassment claims are.

Even more additional: Dr. Evans has made several more comments on the Pharyngula blog; search for comments by “evansd66#3f76d”.

Yet more additional: Bumping the link to the full set of letters from the comments to the main post.

Comments for first half of May 2010

Dr. Becca asked for a montage. I obliged.

It Is Not A Game is normally a political blog, which is not my usual thing to comment on, but I was so pleased that someone else noticed the tendency to express large things in terms of football fields. Which is weird, because there’s a lot of different kinds of football fields...

The birds and the bees and the bats

Dylan Evans is in trouble for discussing animal behaviour.

Several websites, including Pharyngula, Richard Dawkins’s site, and the Huffington Post are running a letter that Dr. Evans wrote describing how he is being sanctioned for showing an article about bat copulation to a colleague.

I had been engaged in an ongoing debate with the colleague in question about the relevance of evolutionary biology to human behaviour, and in particular about the dubiousness of many claims for human uniqueness. I showed it the colleague in the context of this discussion, and in the presence of a third person. I also showed the article to over a dozen other colleagues on the same day, none of whom objected.

HR launched a formal investigation. Despite the fact that external investigators concluded that I was not guilty of harassment, Professor Murphy has imposed a two-year period of intensive monitoring and counselling on me, and as a result my application for tenure is likely to be denied.

There is video of this behaviour online, but as described, this is about showing the article to the colleague. This is arguably the raciest picture in the article:

Now, it’s easy to make jokes about this. (“I’m guessing it was those tawdry error bars that Dr. Evans’s colleague found so upsetting!”) And I’m a reluctant to advocate for a side in a harassment case, because I don’t know the other party’s version of events.

But if the university’s Human Resources department found nothing wrong... why is the President of the institution getting involved?

There is a petition to sign, here:

http://www.ipetitions.com/petition/freedebate/

Additional: The Digital Cuttlefish scores an immediate nomination for Open Lab 2009 for writing all this in verse!

The best education money can buy

There’s a very interesting article in Time magazine about education and experimentation. It describes the efforts of economist Roland Fryer, Jr., to test whether there are ways to pay kids who do well in school.

Now, given that I’ve been reading, and occasionally blogging, about how financial incentives backfire, I was surprised to read that there seems to be some potential in this scheme. But I was even more surprised, and heartened, by the forthright scientific approach taken.

(W)hatever we do, (Fryer) says, we have to test it first — and fearlessly. “One thing we cannot do is, we cannot restrict ourselves to a set of solutions that make adults comfortable.”

Hat tip to the Heath Brothers blog, who point out another interesting thing that the article brings up: we often assume people know how to work on a problem when they don’t. Not quite the Dunning-Kruger effect, but perhaps related.

Original and transformative

Hollywood is looking for original ideas. Given a sufficiently liberal definition of “original,” that is.

“We are always looking for original ideas. As long as they are based on underlying material like a comic book or video game or novel.”

I thought of that attitude when I heard this interview with Craig Venter.

Governments are very risk-adverse in funding new programs. The genome is one of the best examples. ... The irony is people don’t want the government to take risks with their money because they can then consider it’s a bad investment. In fact they’re taking safe bets with the money and we’re not moving forward. So instead of being upset that some programs were funded that didn’t take us anywhere, they should be upset that most of the programs we’re funding have us crawling forward instead of leaping forward.

Many government funding agencies, particularly the National Science Foundation, make a big deal about wanting to fund “transformative” research. I can’t help but think that they want to fund transformative research in the same way Hollywood wants to make “original” movies.

People are risk averse. They don’t want to lose money. So they try to make safe bets by using some sort of proven material. For films, that means books or plays or games; for science, that means small incremental improvements that have gone through peer-review.

A lot of films are made outside Hollywood. There are lots of film festivals where people can show their stuff. In science, though, the number of options to get basic science done outside federal funding seem to be shrinking.

This is just one reason I’m so pleased to see BenchFly’s microgrants program off to a good start, with two projects funded in the first round.

Picture by jurvetson on Flickr, and used under a Creative Commons license.

The beginnings of bone

Bones have probably given us more evidence for evolution than almost anything else. But could bones also pose a problem for evolution?

The Times – not the New York Times, the original, singular Times – interviews frequent quotation workhorse for Texas young Earth creationists, dentist and outgoing State Board of Education member, Don McLeroy.

McLeroy is saying the same things he usually does, but this time, he actually said something a little more interesting if only because it’s more specific and a little less well-trod than usual.

“Take bones,” he says, offering a brief description of the collagen and amino acids in bones as an example of biological complexity. “Intuitively people have a tough time thinking nothing guided this. Are we supposed to believe that all of a sudden, say on April 1, five million years ago, the first bone appeared? The question is, how did evolution do this, and the evolutionists have been painted into a corner. They don’t even have a clue. How did that first piece of bone get there?”

Creationists love asking these rhetorical questions, because they can churn them out quicker than one can answer them. But I am a little surprised to see this particular variation of the question. The last few years, creationists have tended to use the bacterial flagellum when they ask the rhetorical question, “How did it get there?”, since intelligent design made it a cause celebre.

But bone? Bone I haven’t seen as a creationist talking point before. I’m a little pleased to see a slightly different example.

That the example isn’t one that appears in a FAQ on Talk Origins is almost enough to draw attention away from McLeroy’s first bit of misinformation. In posing the rhetorical question, McLeroy erects a straw man. Evolutionary biology does not predict that bone appeared “all of a sudden.” Evolutionary biology predicts that bones originate from previously existing structures and developmental processes, and that these will become more elaborated over geological time.

I went to Google Scholar and typed in “evolutionary origin of bone.” Now, I knew this was going to be a tricky thing to find relevant articles, because I would get a bunch of things about evolutionary origins that are features documented by bones, but probably not about bones themselves. Nevertheless, on the first page, I got three promising hits.

Ruben and Bennet (1987) are really concerned about why vertebrate bones are made of they stuff they are: calcium phosphate rather than what almost all the other species of invertebrate animal use, calcium carbonate. They have some very interesting ideas that are related to the notion that calcium phosphate is more stable under pH conditions that are associated with heavy exercise. But it’s not quite getting at origin of bones.

Smith and Hall (1990) is closer, as they review the origin of bones and other hard structures from an evo-devo perspective. Just reading the abstract makes me feel out of my league:

The appearance of epithelial-mesenchymal interactions and the origin of the skeletogenic/odontogenic neural crest at the outset of vertebrate evolution provided the developmental basis for the evolutionary origin of vertebrate skeletogenic and odontogenic tissues and for the appearance and evolution of the vertebrate skeleton.

Now, putting aside my own ignorance for a second, it’s pretty clear that this alone starts to dent McLeroy’s claim that we “don’t have a clue.” We do have clues – technical and difficult clues – but clues nonetheless.

Finally, I hit the most recent paper of the trio my one Google Scholar search returned by Donaghue and Samson (2002). This, and a later paper by two of the same authors (Donoghue et al. 2006) are the best entry points I’ve found so far. They are both substantive reviews of the origin of the vertebrate skeleton.

McLeroy sort of implies that there is a big divide between the boneless and the boned, but that’s not the case. Indeed, the point of the paper is to point out that it might look like there is a massive gap between vertebrates with a mineralized skeleton and those without a mineralized skeleton if you only looked at living organisms.

This is why you look at fossils. (Of course, McLeroy, as a young Earth creationist, puts no stock in these.)

For instance, Donaghue and Samson point out that while current jawless fish don’t have endoskeletons, many in the fossil record do. Much the same is true with chondrichthyans. Today, that group consists of sharks and ray that mainly use cartilage rather than bone, but in the past, many were more skeletonized.

The paper extensively reviews the fossil record and looks at the variation of skeletons out there, arguing that the earliest vertebrates had an “entirely unmineralized cartilaginous splanchnocranial endoskeleton.” Now the authors point out here and in a later paper (2006) that vertebrates actually have two kinds of skeleton, which complicates matters in answering questions about the origin of bones. But I am guessing that what McLeroy is getting at with “bone” is mineralization.

Using the “related articles” feature from the publisher, I learned – to absolutely no surprise at all – that people are researching the genes involved in mineralization. A family of proteins called secretory calcium-binding phosphoproteins (SCPPs) appear to be critical in mineralizing tissue (Kawasaki and Weiss 2006). And we have plausible evolutionary scenarios for how those genes originated (from an ancestral gene named SPARCL1) and evolved. So even if we were only looking at existing organisms, where the split between vertebrates with and without bony skeletons appears sharper than the fossils show, we have evidence of evolutionary processes the led to the creation of bony skeletons that we have now.

I could go on, but I have other things to do. I just couldn’t quite bring myself to be as taciturn as the Times reporter, who correctly but tersely wrote:

As it happens the evolutionists do have answers — but in a fossil record spanning many more billions of years than a literal interpretation of Genesis allows.

Given that the reporter probably had a deadline, I can understand why he didn’t take the time to do a more detailed refutation. It took me a good chunk of a day to read a few technical papers and try to bang out this quick summary about bone evolution, which is why these rhetorical questions are so effective. Being incredulous is easier than research.

In a sense, McLeroy is right to point out that the evolutionary origin of bones is not a trivial matter. But he’s guilty of poor scholarship (at best) when he says evolutionary biologists are clueless about the origins of bone.

References

Donoghue, P., & Sansom, I. (2002). Origin and early evolution of vertebrate skeletonization Microscopy Research and Technique, 59 (5), 352-372 DOI: 10.1002/jemt.10217

Donoghue, P., Sansom, I., & Downs, J. (2006). Early evolution of vertebrate skeletal tissues and cellular interactions, and the canalization of skeletal development Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 306B(3), 278-294 DOI: 10.1002/jez.b.21090

Kawasaki, K., & Weiss, K. (2006). Evolutionary genetics of vertebrate tissue mineralization: the origin and evolution of the secretory calcium-binding phosphoprotein family Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 306B(3), 295-316 DOI: 10.1002/jez.b.21088

Ruben, J., & Bennett, A. (1987). The evolution of bone Evolution, 41(6) DOI: 10.2307/2409087

Smith, M., & Hall, B. (1990). Development and evolutionary origins of vertebrate skeletogenic and odontogenic tissues Biological Reviews, 65(3), 277-373 DOI: 10.1111/j.1469-185X.1990.tb01427.x

Skeleton by zpeckler on Flickr, used under a Creative Commons license. Figure from Donoghue et al. (2006), Figure 1.

Tuesday Crustie: Shellhead

The town of Cromer, England is apparently very fond of its ties to the sea, and particularly to lobsters. For it is in Cromer that this was spotted:

Fellow comic geeks will know that Tony Stark’s alter-ego is, indeed, sometimes called “Shellhead.” Perhaps not as elegant as the Golden Avenger, but more appropriate in this case.

More pictures of this astonishing mashup here.

Master of Ancient and Forgotten Science: 2

I’ve mentioned before that my buddy John Wick made me a supervillain. The latest game to feature the villainous me is now available at DriveThruRPG. You get a role-playing game for the low price of $5. Come on, you know that fiver would just have bought you a lunch you’d regret later.

Another teacher gets in trouble over evolution

I’m not trying to turn into NCSE Reports here, but I did want to point out this article in Counterpunch about a Weston, Connecticut teacher who wanted to do a class on evolution.

Yeah, you probably know where this is going, don’t you?

A teacher with 17 years experience wanted to do a class that featured Abraham Lincoln and Charles Darwin, riffing off the trivial fact that they have the same birthday. This was submitted to the school’s principal, who basically went all Jack Nicolson in A Few Good Men... on behalf of his students, of course.

(Principal) Dr. Ribbens rejected the proposed program because of its discussion of evolution. ... “I know personally that I would be challenged in leading a 10-year old through this sort of discussion while maintaining the appropriate sensitivity to a family’s religious beliefs or traditions.”

Dr. Ribbens concluded by saying evolution was not age appropriate for Mr. Tangarone’s students and said “TAG topics need to be altered this year to eliminate the teaching of Darwin’s work and the theory of evolution.”

So because the principal can’t figure out a way to do it, it must be impossible. Right?

So, teacher quits, school board says teacher has disciplinary problem, and principal tries apologizing for “heat of the moment” comments.

PubMed vs. Google Scholar

A comment on Twitter about PubMed left me wondering aloud why people use the thing instead of Google Scholar. This idle comment brought a surprising amount of comments.

Before I get to the comments, let me explain my point of view. I’ve never warmed to PubMed, although I know many of my peers use it multiple times daily. I suppose part of it is the “med” moniker. While PubMed does include a lot of the basic biological literature, it’s still fundamentally a medical resource. And I am not, nor ever will be, a medical researcher.

For me, Google Scholar has been the greatest thing since Otto Rohwedder’s invention.^* I always found it much more straightforward to search compared to PubMed’s finicky structure, and it was more likely to just give me the PDF reprint. From what I can see, Scholar searches PubMed, so if I like the interface better, and it includes PubMed, why should anyone use it?

Andrew Thaler was the most explicit Google Scholar booster:

I have never been in a situation where PubMed was better than Scholar.

On the PubMed booster comments, Rob Oakes wrote that Google Scholar includes only part of PubMed index. This I hadn’t known, but found that this has indeed been reported in the literature (e.g., Schultz 2007). Indeed, I hadn’t followed closely, but there are a whole series of articles comparing PubMed and Google Scholar. On a related note, Rob noted that Google Scholar is proprietary, so there’s no clear way to know what it’s doing to bring those hit forward. I’m not entirely sure many people could explicitly spell out what criteria PubMed uses for searches, though. Finally, he said:

Scholar doesn't have any drill-down features. That makes doing medication searches a serious PITA.

I don’t do medication searches, so this is not an issue for me.

eskay8 liked PubMed for integrating with the particular database she uses, while for Jason Goldman PubMed was all about “street cred.”

Biochembelle noted that PubMed’s functions have improved in the last year. She also commented that with PubMed, she didn’t have to “wade through patents and books.” Perhaps she hadn’t seen the new-ish checkbox on Scholar front page that allows you to skip patents.

And that’s perhaps the problem with these services: they’re moving targets. I tried PubMed a few years back and didn’t like it. Others tried Scholar and didn’t like it. But both have moved and changed. An even more recent article found only trivial differences between the two of them.

So Scholar users, maybe it’s worth it to go back and give PubMed another shot. PubMed boosters, try playing with Scholar’s new toys. Maybe you opinions will change. Or maybe JATetro is ahead of the game: he preferred the search engine that Falagas and colleagues (2007) found had features that surpassed both PubMed and Scholar: Scopus.

Additional: Speaking of moving targets, I see that Google Scholar has just added an email alerting function – I think that just went in today, in fact.

Reference

Falagas, M., Pitsouni, E., Malietzis, G., & Pappas, G. (2007). Comparison of PubMed, Scopus, Web of Science, and Google Scholar: strengths and weaknesses The FASEB Journal 22(2): 338-342. DOI: 10.1096/fj.07-9492LSF

Shultz, M. (2007). Comparing test searches in PubMed and Google Scholar Journal of the Medical Library Association : JMLA: 95(4): 442-445. DOI: 10.3163/1536-5050.95.4.442

^* What? You didn’t know the inventor of sliced bread?

Crustacean nociception: Elsewhere

I’ve been shamelessly self-promoting our recent crustacean nociception paper (Puri & Faulkes 2010). But I’m pleased that other people have noticed it, and even written about it. I’m going to use this post to compile responses; if more links come in, I’ll update this post.

The indefatigable Bora Zivkovich gave us featured spot in his regular “New and exciting in PLoS ONE” update. Bora also mentioned the follow-up posts in EveryeONE, commenting:

How I wish more authors blogged about the background stories of their discoveries!

You’re welcome, Bora. ;)

I’m completely tickled that Dr. Scicurious featured the paper on Neurotopia, and talked a bit about the importance of negative results. I think I can say this on behalf of both my co-author and me: Scicurious, we ♥ you! (Her completion of doctorate celebratory ink is pictured; read more about it here.)

Weirdly, Sci’s post came very shortly before another, completely independent post on the paper that first got my name in the scientific literature (if you look very closely) appeared in The Thoughtful Animal blog. So the same day saw fellow bloggers writing about the very first and very latest things in my scientific career. Which was a very odd coincindence.

The Thoughtful Animal was also kind enough to give a plug for the nociception paper under the headings of “Awesome Things, Science and Otherwise.” Right back atcha!

Finally, I’m not above plugging this paper on my other blogs. In particular, I used a couple of posters we gave about this research as examples of things to do – and not do! – over at the Better Posters blog.

Reference

Puri S, Faulkes Z. 2010. Do decapod crustaceans have nociceptors for extreme pH? PLoS ONE 5(4): e10244. doi: 10.1371/journal.pone.0010244

Related posts

• The embargo lifts
• Introducing my co-author
• Origins, Part 1
• Origins, Part 2
• The journal, PLoS ONE
• The worry
• Comparative

Proposed moratorium

Be it proposed that:

All further discussion of he question of whether science is compatible with religion, and all variants thereof, be tabled until such time as there is a general agreement that the many religions of the world, past and present, are compatible with each other.

(Note: This is not a slam of religions. It’s a slam of ignoring diversity.)

Belting it out

Soft. Lost his edge. Past his prime.

Phrases that have never been applied to Iggy Pop.

Many rock ‘n’ roll musicians get mocked for continuing to perform as they get older. But Iggy Pop, in his 60s, is still respected as a rocker. When asked how, he said:

I’m not bald, I’m not fat, and I’m not safe.

I want to be the Iggy Pop of science.

Academics are a bit like musicians: They often do their best work when they’re young, and reach a point where they’re often considered to have lost it and not contributing much new.

Today, I got back one of the tools I need to help keep me on that target. I got my belt back.

Yeah, a belt. Despite my name, I’ve never been very good with non-attachment. I bought this belt – a thick black one with a noisy buckle – when I was doing my Ph.D., and have worn it damn near daily since. Last month, the buckle wore through some on the leather around one of the notches, and I thought I was going to have to replace the belt. But I was able to get just that worn leather replaced, and now it’s almost as good as new.

My appreciation of this belt has gone up the last few years. From time to time, I’ve gotten off the rails, health and living wise. You can’t really ignore it when you’re notching the belt up two notches from where you usually have.

And I’m pretty damn proud that today, the belt is on the same notch it was on in grad school. And I still have hair on my head. And I’m still working on my goal for the year.